Advances and Main Results

Laboratory of Plant Cell Biophysics and Biochemistry

Important fundamental theses:

  • The conception of chlorophyll (Chl) biosynthesis sites (CBS) was advanced and substantiated.
  • Chl metabolic heterogeneity in plant was proved.
  • Turnover of Chl molecules occurred within the whole life of green leaf was revealed.
  • Formation of Chl b from Chl a molecules was established. The possibility Chl b to Chl a intertransformation was shown.
  • The coupling between Chl biosynthesis and chlorophyll-protein complexes of photosynthetic apparatus was revealed.
  • Negative feedback regulation of Chl biosynthesis was shown.
  • New insights on regulation of 5-aminolevulinic acid (ALA), a key precursor of plant tetrapyrroles, biosynthesis was developed.
  • A principal possibility was shown for using Chl biosynthesis as a target of new generation herbicides (photodynamic herbicides).
  • Porphyric precursors of chlorophyll were shown to accumulate in plastids in a monomeric state, being energetically isolated from carotenoids as well as being effective inducers of photodynamic processes in vivo.
  • Fundamental principles of plan resistance to photooxidative stress induced by endogenous porphyrins were established.
  • The coupling was revealed between the activity of magnesium site of plant tetrapyrrol biosynthesis and that of ALA-forming unit.
  • Plant cell natural metabolite (ALA) was shown to exhibit properties of plant growth and development regulator.

Developments and applications:

  • Method for woodlouse control in rosaries was developed and applied in practice of flower-greenhouse enterprise in Minsk.
  • Method for combined cultivation of Chlorella and Chlamidomonas was developed and passed to the collective farm “Pirevichi” for application as a cattle feed additive.
  • Method for food dye production from berry manufacturing waste products as well as technology for its application in meat carcasses stamping was developed.
  • Compact and economically efficient lighting device was developed and manufactured for cultivation of photosynthesizing microorganisms and plant tissues in small bulk.
  • Method based on plant treatment with cytokinins before mowing was developed for increasing nutritive value of rude vegetable fodders.
  • Treatment method based on culturing of unicell algae in potato manufacturing sewage was developed.
  • Order for Spirulina biomass production in laboratory conditions was elaborated.
  • Chlamidomonas pigment mutants (effective protoporphyrin IX producers) were made.
  • Original compositions of photodynamic herbicides were created for dicotyledonous weed control in cereal crops.
  • Technology for intensified root forming on cuttings from some ornamental plants was developed using physiologically active compounds of Chlorella growth medium and peat extracts.
  • High efficiency method for photobiological production of hydrogen from microorganisms was developed.
  • Technology for growing algae on sludge waste of IC “Belaruskaly” was developed in order to reduce prime cost of biomass production.
  • Wasteless and cheap technology was developed for production of biologically active feed additive based on algae (Chlorella) suspension and used in addition to feeding rations of cattle and poultry.

Instrument Engineering:

  • Instrument was designed for determining of hydrogen concentration in gas mixture enabling measurement of microorganism hydrogen-forming productivity.
  • A laboratory model of computerized instrument of PAM- fluorometer type was designed to monitor photosynthetic activity in vegetating plants.
  • Instrument for detection of herbicides residual amounts in soil and agricultural products is being designed based on biosensor developed in the Laboratory.