Team: Liposomal forms of drug preparations and ozonobiology

Laboratory of Biophysics and Engineering of Cell

Team: Liposomal drugs, ozonobiology and cell diagnostic

Head of the team – M.A. Martynova, Ph.D.

Main research trends:

  • Determination of general regularities for formation of liposomes containing medicinal agents. Development of methods for producing liposomal drugs taking into consideration peculiarities of medicinal substances.
  • Developing of ozone technologies for products disinfection based on studying molecular-membrane mechanisms of the ozone effect on microorganisms and other biological objects.
  • Developing technology of determination of breast cancer cells sensitivity in primary culture to antitumor drugs with aim of choosing of optimal chemotherapy.

A method for producing a new medicinal form of an antituberculous antibiotic-liposomal rifampicin for inhalation with a high level of drug inclusion was developed. Component composition and technique of liposome formation, enabling production of a stable form of liposomal rifampicin under the efficiency of antibiotic inclusion to 80%, were installed.
It is designed of the method for incapsulating antiviral drug “butaminophen” into liposomes. Optimum conditions were determined for formation of liposomal butaminofen by mechanic and ultrasound dispersion methods, by multiple freezing-thawing in the presence of cryoprotector and detergent dialysis methods allowing production of liposomal drug for external, peroral and injection application.
Techniques for inclusion of a fatty acid complex “bien” into liposomes are being developed for creating a synthetic analogue of pulmonary surfactant. The liposomal “bien” was shown to increase greatly (no less than by 75%) phagocytic activity of alveolar macrophages.
It is designed of the method for incapsulating β-carotine into liposomes for animals.

Development of liposomal form of medicinal antineoplastic paclitaxel is presently conducted.

A principal new method was developed for producing adenosine-5’-triphosphate (ATP) by biosynthesis with yeast owing to endogenic precursors. Adenosin-5’-triphosphate synthesis is initiated by the ozone effect on yeast for 30-60 min, followed by ATP accumulation in an extracellular water phase that simplifies the procedure of extracting and purifying a product. With the developed method for ATP production, it’s yield from yeast reaches 2.0-3.5 g/kg dry weight of cells. For comparison: ATP yield is 1.2-2.0 g/kg under it’s extraction from the muscular tissue of animals.

General regularities of the ozone effect on microorgamisms and surface cuticular structures of fruits and vegetables were revealed. The ozone effect was shown to be of biphase character: low O3 doses (to 1 mg/m3) stimulate growth and development of microorganisms, high ones (from 3-5 mg/m3 and above) exert bactericidal and fungicidal impact through damage of plasmic membranes and disorder in cell ion homeostasis.
Ozone, in the used dose scale, interacts only with surface membrane structures of cells (including fruit and vegetable) and does not penetrate into their volume.
Multiple short-term treatments of various kinds of products with low ozone doses result in modification of a structural and functional state of curicular fruit formation causing:

  • emergence of gas-selective properties of surface tissues;
  • decrease in water evaporation rate;
  • retardation of metabolic process and reduction in bioenergy losses as a result of “self-burning” of valuable nutrient substances;

    Ozone-treatment of vegetables in the postharvest period results in long-term (months) increase in the activity of their immune system.
    A method for storing fruit and vegetable by treatment with ozone-air mixture was developed on the basis of revealed regularities.

    The methods of primary cultures of breast cancer cells caring in macro- and microamounts from surgery and bioptic samples were developed. Optimal biochemical parameters which characterized influence of using drugs on cell enzyme activity, prolifiration and apoptosis were determined.
    Cultivation of tumor cells without drugs provided estimation of cell dividing rate which reported malignancy degree.
    A method for monitoring the long-term results of treatment of cancer patients by assessing the activity of blood enzymes has been developed .

    The team was developed of laboratory technological regulations for liposomal rifampicin and liposomal butaminofen. The method of fatty acid complex “bien” incorporation into liposomes has been devised.

    The technology a peelings and preparation the drinking water «Akvadiv» before the flow into a bottle was design by means of selection ozone treatment regimes. This technology is used in goods production.

    «Liposomal drugs, ozonobiology and cell diagnostic» team is ready for collaboration on national and international level in area of liposomal drugs design, the development of ozone technologes for water, fruits and vegetables, grain storehouse, equipment disinfection and developing technology of determination of cancer cells sensitivity in primary culture to antitumor drugs. Collaboration is possible as joint projects, agreement for the development of ozone treatment regimes.

    Responsible person - Dr. Maria Martynova (phone: 284-23-61, e-mail: )